The expression of IL-2 and IL-4 in CD4+ T cells from mouse lymph nodes and spleen during HSV-1-induced facial palsy

The expression of IL-2 and IL-4 in CD4+ T cells from mouse lymph nodes and spleen during HSV-1-induced facial palsy

Lintao Gu, Yuechen Han, Wenwen Liu, Yanyan Mao, Jianfeng Li, Haibo Wang

Objective: Herpes simplex virus 1 (HSV-1) is regarded as an important underlying cause of Bell’s palsy, but the immunologic mechanism remains unknown. Here, we employed a mouse facial paralysis model to investigate the expressions of CD4+ T lymphocytes and interleukin (IL)-2 and -4 in the left draining cervical lymph nodes (LCLN) and spleen, as well as the inhibitory effects of glucocorticoids (GCs).

Methods: HSV-1 was inoculated into the surface of the posterior auricle to generate the facial paralysis model. The paralyzed mice were divided into three groups; in one group without any treatment, mice were killed at different time points, and those in the other two groups were injected with methylprednisolone sodium succinate (MPSS) or with a combination of MPSS and GC receptor blocker (RU486). The expression levels of CD4+ T lymphocytes and CD4+-IL-2+ and CD4+-IL-4+ cells in the LCLN and spleen were detected by fluorescence-activated cell sorting.

Results: Expression levels of CD4+, IL-2, and IL-4 first increased then decreased in LCLN and spleen and peaked 5 and 7 days, respectively, after the manifestation of facial paralysis. All the data at the peak points were significantly different compared with control (p < 0.05), and these effects were inhibited by MPSS.

Conclusion: Our results suggest that CD4+, IL-2, and IL-4 participate in the HSV-1-induced facial paralysis immune response. MPSS can effectively attenuate HSV-1-mediated nervous system damage, which is associated with its inhibitory effect on expression of these inflammatory markers.